NMR provides evidence for dynamic hydrogen bonding in proteins
نویسندگان
چکیده
منابع مشابه
NMR detection of side chain-side chain hydrogen bonding interactions in 13C/15N-labeled proteins.
We describe the direct observation of side chain-side chain hydrogen bonding interactions in proteins with sensitivity-enhanced NMR spectroscopy. Specifically, the remote correlation between the guanidinium nitrogen 15Nepsilon of arginine 71, which serves as the hydrogen donor, and the acceptor carboxylate carbon 13CO2gamma of aspartate 100 in a 12 kDa protein, human FKBP12, is detected via the...
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The structural, vibrational, and energetic properties of adducts of alkanes and strong cationic proton donors were studied with composite ab initio calculations. Hydrogen bonding in D-H(+)...H-alkyl adducts contributes to a significant degree to the interactions between the two components, which is substantiated by NBO and AIM results. The hydrogen bonds manifest themselves in the same manner a...
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On the basis of experiments at 275 GHz, we reconsider the dependence of the continuous-wave EPR spectra of nitroxide spin-labeled protein sites in sensory- and bacteriorhodopsin on the micro-environment. The high magnetic field provides the resolution necessary to disentangle the effects of hydrogen bonding and polarity. In the gxx region of the 275 GHz EPR spectrum, bands are resolved that der...
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In biomolecules, bifurcated H-bonds typically involve the interaction of two donor protons with the two lone pairs of oxygen. Here, we present direct NMR evidence for a bifurcated H-bonding arrangement involving nitrogen as the acceptor atom. Specifically, the H-bond network comprises the Nδ1 atom of histidine and both the backbone N-H and side-chain Oγ-H of threonine within the conserved TXXH ...
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Background and Objectives: Visit length is a crucial aspect of patient-physician relationship that its inadequacy can negatively influence the treatment efficiency. Studies on visit length and the factors influencing it are limited in developing countries. Thus, the objective of this study was to survey this variable at provincial level, and provide data on factors affecting it. Methods: A ...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2007
ISSN: 0961-8368,1469-896X
DOI: 10.1110/ps.072945407